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Use of active extracts of poplar buds against Penicillium italicum and possible modes of action

注意:本論文已在Food Chemistry 196 (2016) 610–618發表
使用者請注明論文出處

Shuzhen Yang a, Limei Liu a, Dongmei Li b, Huan Xia a, Xiaojun Su c, Litao Peng a,?, Siyi Pan a
a Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China
b Georgetown University Medical Center, Washington, DC, USA
c Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Collaborative Innovation for Utilization of Botanical Functional Ingredients,
Hunan Agricultural University, Changsha, 410128, China


Abstract:
Antifungal components, from poplar buds active fraction (PBAF) against Penicillium italicum, the causal agent of blue mold in citrus fruits, were identified and possible action modes were investigated.
Pinocembrin, chrysin and galangin were determined as active components in PBAF, using HPLC and HPLC–MS analysis. The antifungal activity is stable at temperatures ranging from 4 C to 100 C and
pH levels ranging from 4 to 8. In the presence of PBAF, the hyphae become shriveled, wrinkled and the cell membrane became seriously disrupted. Further investigation on cell permeability, nucleic acid
content and alkaline phosphatase suggest that the cell membrane might be the target. Mycelial oxygen consumption and the respiration-related enzymatic activity of succinate dehydrogenase, malate dehydrogenase and ATPase were all inhibited by PBAF. We propose that PBAF is a potentially useful alternative for blue mold control and may act against P. italicum by interfering with respiration and disrupting the cell membrane.
Keywords:Poplar buds; Active constituents; P. italicum; Mode of action; Respiration

摘要:對柑橘青霉病病原菌,意大利青霉表現抗菌作用的楊樹芽中提取物中的抗真菌活性成分鑒定出來,并且調查了其可能的作用機制。采用HPLC和HPLC-MS分析,楊樹芽活性組分中的抗真菌成分確定為松鼠素、白楊素、高良姜素。抗真菌活性在4-100度下,pH?4-8條件下穩定。在存在楊樹芽活性組分時,真菌扭曲、收縮、細胞膜結構嚴重破壞。進一步分析細胞通透性、核酸含量、堿性磷酸酶活性發現細胞膜可能是作用靶標。菌絲的呼吸好樣和呼吸代謝相關酶琥珀酸脫氫酶、蘋果酸脫氫酶和ATP酶均被楊樹芽活性組分抑制。我們認為楊樹芽活性組分是控制柑橘青霉病的潛在替代方法,其可能通過干擾呼吸和破壞細胞膜結構抑制意大利青霉。

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本站收錄的本文作者的其他論文:

1、 Effects of poplar buds as an alternative to propolis on postharvest diseases control of strawberry fruits
2、“法蘭地”草莓果實中花色素苷的組成及穩定性
3、松針提取物抑制柑橘青霉病菌作用及活性成分分析

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